Hands on: BASIC Techniques needed in STEM CELL Isolation and culture

3-Day Cell Culture   
The purpose of the Cell Culture and Stem Cell Research Workshop is to provide a solid understanding of basic cell culture techniques needed to proceed with stem cell research through classroom and laboratory work. Attendants will gain significant experience in the manipulation and isolation of cells through culture. Through integrated learning techniques, utilizing hands-on training, and lectures. Attendants will learn the biological basis and relevance of the process of culturing cells as well as the basis of stem cell research. This will allow interested attendants to translate the techniques and information learned in this workshop into their own laboratories

This workshop provides prerequisite training for the culture of numerous cell types.

Who should attend?

Personnel in the fields of Immunology, Virology, Microbiology, as well as all laboratory personnel dealing with in-vitro fertilizations, and tissue typing. Also, pharmaceutical laboratories personnel and members of the Egyptian Society of Immunology.

Course Programme

09:30 AM – 11:30 AM Lectures

11:30 AM – 12:00 PM  Break

12:00 PM – 04:00 PM  Practical work

A certificate will be awarded upon completion of the course

Topics Covered

1)    Introduction to cell and tissue culture.

2)    Serum-containing and Serum-free culture media.

3)    Trypsinization.

4)    Cell counting, viability and growth curves.

5)    Growth of adherent and non- adherent cells.

6)    Suspension culture differentiation and attachment.

7)    Adaptation to suspension culture.

8)    Adaptation to suspension serum-free media.

9)    Cryo preservation and cryo thawing.

10)Primary culture and the cell cycle.

11)Stem Cells (types & origins).

12)Stem Cell Separation.

13)Mesenchymal stem cells.

14)Cord blood transplantation.

Speakers

Teaching Staff of the Medical Research Institute, Alexandria University.

Registration Fees

1200 LE for Egyptian University Staff Members

500 $ for Non-Egyptian

Accommodation

is available upon prior request of at least one month before the course start date:

125 LE for Single room/ day

200 LE for Double room/ day

For further enquiries, please contact

Mrs. Mai Kotb     Tel: 01063436912

Add: 71 Vector Emanuel St., Smouha, Alexandria

Phone : 03-4266213/17/18     FAX : 03-4266214

E-mail : This email address is being protected from spambots. You need JavaScript enabled to view it.